Thursday, November 28, 2019
#InsertTitleHere free essay sample
Welcome back to the black-hole time-sucking wasteland known as Youtube, HipsDontLie6969! Itââ¬â¢s been an eternity since weââ¬â¢ve last seen you. What, a whole two days? Unacceptable. You have 53 videos piled up in your subscription boxââ¬â a record high! How have you managed to survive without staring at Michelle Phan slap makeup on for five minutes or watching yet another adorable puppy video? No, donââ¬â¢t look away from the screen. You still need your daily dose of prank-gone-wrong videos and low-budget trailers for movies youââ¬â¢ll never see. There we go, two hours youââ¬â¢ll never get back! Oh, but youââ¬â¢re not done yet. In fact, weââ¬â¢ve barely just started! To tell you the truth, weââ¬â¢ve been worried about you. First you havenââ¬â¢t posted an Instagram picture in three days, now youââ¬â¢re talking with your friend and havenââ¬â¢t checked your phone in over five minutes. We will write a custom essay sample on #InsertTitleHere or any similar topic specifically for you Do Not WasteYour Time HIRE WRITER Only 13.90 / page Whatââ¬â¢s next, no more selfies every three seconds?Donââ¬â¢t make Siri ask you whatââ¬â¢s wrong. This is getting incredibly unhealthy and youââ¬â¢re showing signs of all the symptoms: being attentive to other people, engaging in conversation, good god, even LOLing (or as the prehistoric barbarians used to call it, ââ¬Å"laughingâ⬠). Iââ¬â¢m afraid you have a severe case of the ââ¬Å"Lifeââ¬â¢s So Much Better Not Looking Through A Screen But Actually Enjoying the Present Syndromeâ⬠. Iââ¬â¢m afraid recovery is not going to be an easy process. People complain about recovering from cancer or a surgery, yet they donââ¬â¢t understand the amount of work it takes to find a post-worthy selfie with just the right cleavage-to-duckface ratio. Then you need to find an adequate (usually irrelevant) caption, maybe another Taylor Swift lyric or movie quote? How about a good ââ¬Ëole hashtag? ââ¬Å"#selfiesunday #hashtag #love #girl #boobsâ⬠it is . Oh, donââ¬â¢t even get me started on the filters. Yet the hardest part of healing is relearning the unspoken rules of ââ¬Å"text languageâ⬠: to be able to typeââ¬Å"HAHAHAHAHAHAHAHAHAHAâ⬠without a remnant of a smile on the face or decimate the English language so u tlk lik dis and sound rly smart lol:) Recovery is also hard on your loved ones, as you slowly sink back into to the habit of taking pictures of your food at the dinner table instead of eating it or texting while your Mom has, or at least attempts,a one-sided conversation. This journey back to health will be full of glazed eyes and relapses back to reality, but #webelieveinyou. We see a large cause of this sickness is due to that boy youââ¬â¢ve been flirting with in math class. Donââ¬â¢t think we havenââ¬â¢t noticed like Google, we know everything. Also like Google, weââ¬â¢re here to help and make sure you donââ¬â¢t have to use your own brain for anything. If you would like to talk to him, text him instead! Everyone knows talking online is the same if not better than in person. You get to filter your true thoughts and hide behind a rampage of smiley face emojis. If that doesnââ¬â¢t work out, welcome to Tinder! With just a swipe right, this mobile dating app matches you with sweet sentimental guys who are looking for nothing short of a lifelong romance (long walks on the beach and kisses in the sunset not included). Next, weââ¬â¢ll prescribe a strong dose of Twitter; make sure to take this at least once an hour. After all, everyone is dying to know where you are, what your mood is, and what youre wearing at all times, Now donââ¬â¢t forget, if you want to heal faster, make sure to also subtweet your ex-boyfriends new girlfriend around once a week, because who doesnââ¬â¢t love a good twitter fight.Sure you could be paying attention in class, but Nicoleââ¬â¢s status on the cloudy weather seems much more important . Favorite. As the saying goes, pictures/videos or it didnââ¬â¢t happen. This treatment is one of of the best cures towards your diagnosis. What would be the point of going to a concert if you didnââ¬â¢t take a video? Concerts used to be about the live experience, the intimate connection between artist and fan, and the swarming energy that causes people to dance, laugh, sing along. Never mind that when now you can capture it and just re watch it again later. This applies when you hang out with friends as well. Make sure to take as many pictures as possible so you can brag about your friendship through ââ¬Å"candidâ⬠laughter photos and Snapchat storiesthen you can go back to ââ¬Å"hanging outâ⬠, aka being in each otherââ¬â¢s presence as you both stare away at your phones. Have you ever walked into a silent room where everyoneââ¬â¢s heads were craned down, scrolling through their Facebook newsfeed? Satisfying, isnââ¬â¢t it? Silence speaks louder than words. You seem to be doing much better pale ghastly skin, a sore neck from constantly looking down, and the inability to go to the bathroom, or anywhere really, without your phone seemingly attached to your hand. You used to make small talk with the stranger in the elevator or only Facebook friend people you really knew, but fortunately weââ¬â¢ve successfully removed those malignant actions. Now youââ¬â¢re a living success story: socially impaired with+800 Facebook friends who couldnââ¬â¢t give a rats ass about you! With our current treatment, less and less people are getting struck with this contagious virus. Luckily, we are starting vaccinations at a younger age, with little kids playing on smartphones instead of outside and taking bathroom mirror pictures before theyââ¬â¢re tall enough to reach it. By the next five years, kids are projected to have smartphones handed to them right out of the womb! In the age of technology and advancement, those struck down with the syndrome are dying out while the rest of the population is progressing forward. Iââ¬â¢m glad you were able to recover and join us.Make sure to follow the necessary steps to prevent the ââ¬Å"Lifeââ¬â¢s So Much Better Not Looking Through A Screen But Actually Enjoying the Present Syndromeâ⬠from resurfacing. Have as many social media applications as possible and when in doubt, never look up. Your screen is your reality. If youââ¬â¢re reading this off paper (does that still exist?), youââ¬â¢re doing it wrong. Oh , well pardon me, I forgot to look at the time! Itââ¬â¢s that time of the day again. Welcome back to the black-hole time-sucking wasteland known as Youtube, HipsDontLie6969! Itââ¬â¢s been an eternity since weââ¬â¢ve last seen you. What, a whole two hours?
Sunday, November 24, 2019
President Bush Lays Down Plans essays
President Bush Lays Down Plans essays The State of the Union is a speech delivered by the president directed towards Congress and the citizens of the United States. In this speech, the president addresses the problems that our nation faces today; both domestic and foreign. President George W. Bush opened his speech with the issue of America's economy. He proposed an income tax reduction that would save a family with a $40,000 income of approximately $1,000. This tax cut would help small businesses throughout the country. He also wants Congress to pass a law that would stop the unfair double taxation of dividends. This would help investors out and in turn strengthen the economy. Another issue stated was to have a reliable health care system. Many citizens are not fortunate enough to have money to pay for health insurance. He wants the government to give financial support to those in need of health care. Also, President Bush wants to pass a reform that helps protect doctors from frivolous lawsuits. These lawsuits are driving doctors to charge more for their service. The third goal that the president wanted to accomplish was improving the environment. He set a comprehensive energy plan to promote energy efficiency and conservation. He passed the Clear Skies legislation that mandates a 70-percent cut in air pollution from power plants over the next 15 years. Also, he proposed $1.2 billion in research funding for hydrogen-powered cars. Being more efficient and cautious of the environment will preserve our country. He also addressed many social problems that Americans face today. Many children are left with nobody to look up to due to parents being in prison. He urged people to be mentors at schools for children who are not so fortunate. Also, he wants to pass a faith-based initiate and Community Service Act to fund groups that help out the needy. He set up a program that gives 600 million dollars to help pay for drug rehabilitation for Americans to receive the treatment ...
Thursday, November 21, 2019
International criminal law Essay Example | Topics and Well Written Essays - 2000 words
International criminal law - Essay Example urder or conspiracy to kill members of a particular group with the aim of eradicating that group in totality.1 For genocide to qualify as a crime it must have two elements namely; the physical element and the mental component. The physical component involves committing five acts that are stipulates in Article two of the Convention that include; forcibly transferring children of a particular group to another one, killing members of a certain group, imposing measures or rather sanctions that are of the intent of hindering births within a particular group and causing mental and bodily torture to members of a group. The mental element is primarily based on the intent to destroy partly or in whole a religious, national, ethnic or racial setting. 2 In order for a crime to be regarded as genocide, it has to satisfy the provision of the two elements to make the perpetrators culpable under the internal criminal law. Both factors are crucial in forming the premise of genocide as a crime since none of them is independent, and thus they have to be satisfied to substantiate the crime. 3 Article three of the Genocide Convention outlines five distinct acts that are punishable in the context of the crime of genocide. These acts are as follows; complicating in genocide, attempt to commit genocide, genocide, direct and public incitement to commit genocide. When the actions outlined in this article are combined with the actual acts described in Article two of the Convention the result is the crime of genocide. Under the Convention of Genocide, the law protects four particular groups whose execution should amount to genocide. A religious group involves one whose members share common religious practices and beliefs. An ethnic group is that whose members can be identified by a common language or any other cultural identities. A racial group is primarily people who can be defined by standard physical characteristics for instance; skin colour. A national group is composed of individuals
Wednesday, November 20, 2019
Human Resource Management Essay Example | Topics and Well Written Essays - 2500 words - 4
Human Resource Management - Essay Example Many companies do not have enough resources to invest in the recruitment process. As a matter of fact, for a company to attract the most suitable candidates they need to advertise as much as possible and the most cost effective and efficient way is that of web based recruitment. It is very important to communicate properly and have well written descriptions. To attract the potential candidates it is significant to have a well written description of the position which is neither too long nor too short. It should properly communicate all the important information. Introduction A process of attracting the most suitable candidates and making them apply for that particular job in a company or organization is known as recruitment. It is a process and a way to hire the right type of candidates for the right job. Recruitment is becoming a very critical and significant component of the human resource structure (Alvesson, 2010). It is highly significant to hire people who have more qualities a nd are more talented. For this a very fine and properly tuned up recruitment process is required. This is to ensure that the hired people are the most talented ones and are the best fit for the job. In order to measure the effectiveness of the recruitment process the organization needs to give importance to the feedback from internal customers, its employees and from the external customers. There are several methods of recruitment process. The two broad headings for the recruitment method are that of internal recruitment and external recruitment (Becker, 2007). Job advertisement, recruitment and employment agencies, internal bulletins and the recommendations personally, job centers of government and the new social media are the few most commonly used methods for recruitment. Job advertisement is the most widely used recruitment method. In this method the organization critically and carefully sought out the places it needs to advertise in. A typical job advertisement includes all the important information like job title, the location, description, compensation package and instructions to apply for the job (Thompson & Luthans, 1990). Organizations at times use the agencies like recruitment agencies and employment agencies to attract the most suitable candidates and hire them. The agencies help the organization in the screening of the resumes in the initial phase, the assessment of qualifications and their test, and also the reference checks. Personal recommendations and internal bulletin is also a method of recruitment (Sheridan, 2002). This is done by the organizations to alert the staff for the available positions. The organizations use this method to put up an incentive for the ones who are in search of advancement and also to seek the employee referrals. This method can be an effective one as the employees are already associated with the company and helps in saving the time and money of the company. Another method is job centers of government that usually ad vertise the job for the development of employees in an effort to serve the citizens in search of employment. The new and the latest way of recruitment is that of social media. This is the fastest and the most fruitful resource for recruitment these days. It helps the professionals connect all round the globe. This method is very efficient in attracting the maximum number of candidates in the shortest possible time (Wanous
Monday, November 18, 2019
Teaching Reading to Students with Learning Disabilities Essay
Teaching Reading to Students with Learning Disabilities - Essay Example The development of effective reading strategies can have positive consequences for students with learning disabilities, yet many students do not learn to use strategies automatically. Finally, the implications for reading instruction for students with and without disabilities in inclusive settings are discussed. The ability to read is a critical component of school success. A strong correlation exists between poor reading ability and school failure, and students who do not learn how to read during their elementary years have difficulty navigating the school curriculum during middle and upper grades (Marston, Deno, Dongil, Diment, & Rogers, 2005). The National Center for Education Statistics reported that more than 75% of all fourth graders and eighth graders scored below the reading proficiency range on the most recent National Assessment of Educational Progress (NAEP) reading assessment (U.S. Department of Education, 2002). These numbers suggest that a large number of students with and without disabilities are not benefiting from current traditional reading curricula and instructional practices (Carnine, Silbert, & Kameenui, 2001; Simmons & Kameenui, 2000). Improving reading instruction is a top priority for both general and special education. These concerns are paramount for students with high-incide... Among the students with high-incidence disabilities, children classified with learning disabilities constitute the largest group of students receiving special education services. More than 2 million students in the United States are classified with a learning disability, a number that has increased dramatically since the late 1970s. In the past decade alone, the number of students classified with a learning disability increased 38%, and currently students identified with a learning disability represent 51% of all students with disabilities (U.S. Department of Education, 2000). The 2001 reauthorization of the Individuals with Disabilities Education Act (IDEA) specifically identified the general education setting as the most appropriate placement for all students, and about half of the students classified with a learning disability participate full time in a general education classroom. Full-time placement in the general education classroom is commonly called inclusion or inclusive education (U.S. Department of Education, 2000). In inclusion settings, students with disabilities may or may not receive additional support from a special education teacher. Some programs involve general and special education teacher collaboration, but in many cases the general education teacher is responsible for implementing all academic support services, curricular adaptations, and testing modifications (Schulte, Osborne, & Erchul, 2002). Moreover, most new standards-based reform initiatives require that students with learning disabilities meet minimum local and state competenc y requirements. Despite the extra responsibilities this places on teachers, many school districts continue to reshape the roles of
Friday, November 15, 2019
Role Of Employee Attitudes And Perceptions Business Essay
Role Of Employee Attitudes And Perceptions Business Essay Employee attitude and perceptions play a very vital role in the productivity of the organisation. Overall the employee s behaviour and attitude can vary based on a number of factors such as job satisfaction, leadership, rewards, performance appraisal and various other motivating factors. This research paper aims to look into these factors about the employee attitude and how can it affect the organisation. 1.1 Background Study The employees are the main force for any organisation and it is these employees who decide the running of the company in many cases when it comes to productivity and achieving the goals, without this human force the organisation is just nothing, it is this force which makes use of the raw materials and turns them into a furnished products or goods. But when we speak about the attitude and the nature of the employees it may differ from person to person, because each individual has his own abilities, learning experience, innovative thinking and so on and by this they bring in different kinds of aspirations into the job (Mullins 2002) and apart from this they are of different gender and age, perceptions etc which will lead to a different behaviour from each of them and hence due to this sometimes there may be rational thinking as well , so here it is clear that individual values do matter and how this can be related to job satisfaction and their performance which can indirectly affect the organisation. There are a few techniques in management which can be used to investigate the perceptions and behaviour of employees in the organisation and also some theories which we will touch upon. 1.2 Research Aim Objectives 1. This research paper aims to investigate the employee behaviour and their attitude with respect to leadership 2. The paper also aims to investigate whether employee attitude and job satisfaction go hand in hand 3. Can the employee attitude be changed which is also referred to as OB MOD 1.3 Research Question The attitude of the employees towards work in the organisation and what effect it can have in the organisation 1.4 Keywords: Organisation behaviour, employee satisfaction, attitude, perception 1.5 Area of Research: Employee Attitude, job satisfaction (Organisation Behaviour) 2.0 Literature Review Genetic research suggests that 30% to 40% of job satisfaction is inherited. Job satisfaction and life satisfaction are interrelated and influence each other. A gallop poll indicates that approximately 10% to 13% of workers are dissatisfied, while about 85% of workers are satisfied. Other surveys asking questions in a different manner suggests that more workers are dissatisfied. Job satisfaction and performance varies with the type of occupation, for example, higher management means more satisfaction. Personal characteristics of workers also have an impact on job satisfaction. Job satisfaction increases age. The level of education is slightly negatively related to job satisfaction. If personal skills and abilities are not required by a job, job satisfaction decreases. When a person is more adjusted personally, they will be more satisfied with work. Most of all having a job with decent and fair wage may be the most important variable to job satisfaction. High job satisfaction is associ ated with low turnover and low absenteeism and with high commitment. Although the evidence is not conclusive, high job satisfaction is associated with high performance and pro social behaviour. 2.1 Employee Attitude And Perceptions Employee attitude and perceptions used in research and facet versus global measures. The areas discussed are not meant to provide knowledge of all relevant considerations for designing employee surveys, but rather provide background on the research and an overview of some major areas of study. In the research literature, the two most extensively validated employee attitude and perception towards their job satisfaction survey measures are the Job Descriptive Index (JDI; Smith, Kendall, Hulin, 1969). 2.2 Assessment Criteria for Job Satisfaction The JDI assesses satisfaction with five different job areas: pay, promotion, co-workers, supervision, and the work itself. The JDI is reliable and has an impressive array of validation evidence. The MSQ has the advantage of versatility long and short forms are available, as well as faceted and overall measures. Another measure used in job satisfaction research (e.g., Judge, Erez, Bono, Thoresen, in press) is an updated and reliable five-item version of an earlier scale by Brayfield and Rothe (1951). All of these measures have led to greater scientific understanding of employee attitudes, and their greatest value may be for research purposes, yet these measures may be useful for practitioners as well. In practice, organizations often wish to obtain a more detailed assessment of employee attitudes and/or customize their surveys to assess issues unique to their firm. In the Fisher (2000) study, it suggest, that positive and negative emotions both have a profound effect in predicting overall satisfaction on job. In conclusion, affect while working is a missing piece of overall job attitude. E.g. Twenty-four male and female managerial workers were asked to complete a diary during work hours. The diary was to include a report of the workers the state of mood at four different intervals during the working hours. And this was completed in 16 days. Mood was measured using a 24-item checklist called the Current Mood Report (CMR). The CMR was used to assess the dimensions of pleasantness and activation. At another time, the participants were asked to complete a measure of overall satisfaction using the Valence-Instrumentality-Expectancy (VIE) measure of beliefs about the job. Affect intensity was measured using the Affect Intensity Measure (AIM), a 40-item questionnaire designed to measure individual differences in affective responsively. Dispositional h appiness was measured using two brief self-report measures of general happiness. The results indicated that were obtained from stated the average levels of the employees about their pleasant mood in the sixteen day period and VIE beliefs about the job made important and this contributions from the individuals have a great impact on the overall satisfaction of the job. The purpose of the Weiss, Nicholas, and Daus (1999) study was to investigate the different influences of episodic levels of pleasant mood at work. They examined the relationship between one s job performance and job satisfaction, and prediction of patterns of affective states over time. Weiss et al. predicted that reported mood would be highly associated to general job satisfaction and that individual differences in general happiness would be related to mood levels as well as changes in mood over time. Finally, it was expected that they could discover cycles in mood changes over time. Job empowerment enables employees to participate in decision-making process and experience autonomy across and down the hierarchy. Although in real business, it s easier to have an empowerment policy than to actually implement it (Rosenfeld Wilson 1999:101), it s said that empowerment prorammes can lead to motivated staff, quality customer service and improved profits (Jamison 1999:20) by means of job satisfaction. Self-managed team-working approach relates to job empowerment, and is widespread and increasingly popular in USA (Mullins 2002). Individual members of the group have higher levels of job satisfaction (Cordery Smith 1991:464), because they get greater autonomy and responsibility for the work of team. Also, members of team can work out best means to achieve goals by team effort, and member can improve skills and knowledge by learning from each other during working. Autonomous working groups can be means to enhance productivity, flexibility and work satisfaction. 2.3 Motivation Theories for Improving Productivity and Job Satisfaction Motivation is a complex subject, it is a very personal thing, and it is influenced by many variables. Each and every individual has his own needs and requirements and every one would like to satisfy it in one way or the other.. The various needs and expectations at work can be categorized in a number of ways for example the simple divisions into physiological and social motives, or into intrinsic and extrinsic motivation. Broad three-fold classification as a starting point for reviewing the motivation to work, and also given the complex and variable nature of needs, Economic rewards such as pay, fringe benefits, pension rights, security and other forms of material goods. Intrinsic satisfaction is derived generally from the type of work, the interest shown in the job, and the employee s personal growth and development from various perspectives. The major theories of motivation include Maslow s Theory of hierarchical needs Herzberg s two- factor theory; Alderfer s modified needs hierarchy model s theory; McClelland s theory Often out of these theories Maslows Hierarchy and Herzberg s theory have been discussed a lot by many researchers and has a significant role in the motivation segment of any organisation. 2.4 Previous Study on Motivation An article named What motivates employees according to over 40 years of motivation surveys by Carolyn Wiley aimed to explore the factors that motivate employees in their jobs. The methodology was Secondary survey data. Between 1946 and 1992 surveys were administered by different researchers to people employed in all industries. These were designed to identify the top motivating factors for employees at each particular time. Carolyn Wiley then compares the findings of these surveys. Carolyn discovered that the most highly motivating factor in the workplace has changed over the last 40 years and matured somewhat from recognition for a job well done to the need for a challenge and enjoyment at work. It would be interesting to see if the new research topic can build on these surveys and identify the most highly motivating factor in 2004. It would then be possible to say whether societies needs at work have matured any further since 1992. 3.0 Research Design and Methodology The data required to investigate the specified research area is qualitative form of data. The primary data will be collected mainly via questionnaire and interviews which will be again compared with the available secondary data which is sought from various journals and other online sources of previous works already done by research experts. Thus the data collected through various sources would be analysed later in the next stage for carrying the research to next level and to see how fart eh research is can go ahead. The secondary data would also be used to identify and describe motivation and its importance to the work place, and will attempt to gain an insight into what motivates employees to be successful across a number of different industries and how their attitudes differ. I would especially like to identify the most common motivator to see how this compares with those that have been identified through 40 years of research highlighted by Carolyn Wiley. I would like to see if the most common motivator across the industries has matured further since 1992 when it was found to be the need for a challenge and to enjoy your job. I predict that the most common motivator now will be somewhat more sophisticated. I am also probe my sample of employees into revealing information surrounding their perception of employee benefits such as pension schemes, medical cover etc and whether these things make them perform better at work or make their jobs more satisfactory. It would be interesting to note if em ployees automatically expect to receive such benefits and do not see them as a motivator to increase performance. Thus the data gathered from the questionnaires will be qualitative categorical data. I will analyse this data by firstly reading the completed questionnaires and identifying any that cannot be used. Categorical data cannot be measured numerically but can be classified into sets or categories according to criteria set by those analysing the data. This will have to be done a number of times in order to look at different aspects of the research topic. 4.0 Data Analysis There is no doubt that the data analysis is the most mysterious and complex phase of any qualitative research project, and this is the phase which is least discussed in any literature of a research, many of the strategies which are involved in the research program are familiar to many experienced researchers and it s a crunch time for those who just get in to this practice of research. Generally people start creating a database once they gather any information, however just creating a database is not going to serve the purpose it is s must to turn this raw data into a useful data which can be used for some purpose and gives the scope for advanced research, so this requires an analytical process of the data and there are many software s in the market like Excel and SPSS which can be used for the analysis of the data. SPSS is similar software like Microsoft Excel and is highly used in the data analysis of any research these days. Once the required information is gathered, processing that information is very vital to get the right statistics. Because without analysing the collected data we cannot come to a proper conclusion and there is no use for the research. In our case the target audience of the research are mainly the employees, and the data is to be acquired mainly form the employees working in the organisation where a lot of scope is given for motivation and employee job satisfaction.. 5.0 Time Chart 6.0 Conclusion
Wednesday, November 13, 2019
Womenââ¬â¢s Influence in Medicine :: Essays Papers
Womenââ¬â¢s Influence in Medicine There are many women who had huge influences in the advancement of heath and medicine. Many people donââ¬â¢t realize how much women do and how much they have contributed to the medical world and its advancements. From Lillian D. Wald, who worked with the less fortunate and children in schools, to Virginia Apgar, who worked with mothers and their newborns and also came up with the ââ¬Å"Apgar Score,â⬠and Eku Esu-Williams who is an immunologist and an AIDS Educator. Even though women did so much, many people were sexist and didnââ¬â¢t want to acknowledge what they did or give them the chance to do things, such as become doctors. I want to inform people on how much these women have contributed to the world of healthcare and medicine so that people wonââ¬â¢t be so sexist towards women. There are too many times that the nurses are taken for granted as a part of the school system. In the late 1800ââ¬â¢s and early 1900ââ¬â¢s, the school nurse was completely unknown, even though diphtheria, measles, scarlet fever, chickenpox, and many other eye and skin conditions affected thousands of school children, and not to mention all of the injuries that could occur from day to day at school, in class or during recess. But, thanks to Lillian D. Wald and her visions, efforts, dreams, companions, and her hard work, the situation in most schools changed. In 1902, the school-nurse program began to succeed, and it was one of the very first steps in the development of the public-health nursing system in the United States. Lillian Wald was born in Cincinnati, Ohio, on March 10, 1867 into a cultured Jewish family. Both of her parents were immigrants, her mother was from Germany and her father was from Poland. The Waldsââ¬â¢ moved from Cincinnati to New York where Lillianââ¬â¢s father, Max, dealt in optical wares in Rochester. She had the advantage of a very good education; not only did she know Latin, but she also spoke German and French as well as English. By the time she reached the age of 21, Lillian felt that she needed secure work because she didnââ¬â¢t have any plans for marriage. To try to fill the need she had felt, Lillian chose nursing. She enrolled into the New York Hospital Training School for Nurses, and after finishing the two-year program at the Nursing School in 1891, she took a position at the New York Juvenile Asylum. Womenââ¬â¢s Influence in Medicine :: Essays Papers Womenââ¬â¢s Influence in Medicine There are many women who had huge influences in the advancement of heath and medicine. Many people donââ¬â¢t realize how much women do and how much they have contributed to the medical world and its advancements. From Lillian D. Wald, who worked with the less fortunate and children in schools, to Virginia Apgar, who worked with mothers and their newborns and also came up with the ââ¬Å"Apgar Score,â⬠and Eku Esu-Williams who is an immunologist and an AIDS Educator. Even though women did so much, many people were sexist and didnââ¬â¢t want to acknowledge what they did or give them the chance to do things, such as become doctors. I want to inform people on how much these women have contributed to the world of healthcare and medicine so that people wonââ¬â¢t be so sexist towards women. There are too many times that the nurses are taken for granted as a part of the school system. In the late 1800ââ¬â¢s and early 1900ââ¬â¢s, the school nurse was completely unknown, even though diphtheria, measles, scarlet fever, chickenpox, and many other eye and skin conditions affected thousands of school children, and not to mention all of the injuries that could occur from day to day at school, in class or during recess. But, thanks to Lillian D. Wald and her visions, efforts, dreams, companions, and her hard work, the situation in most schools changed. In 1902, the school-nurse program began to succeed, and it was one of the very first steps in the development of the public-health nursing system in the United States. Lillian Wald was born in Cincinnati, Ohio, on March 10, 1867 into a cultured Jewish family. Both of her parents were immigrants, her mother was from Germany and her father was from Poland. The Waldsââ¬â¢ moved from Cincinnati to New York where Lillianââ¬â¢s father, Max, dealt in optical wares in Rochester. She had the advantage of a very good education; not only did she know Latin, but she also spoke German and French as well as English. By the time she reached the age of 21, Lillian felt that she needed secure work because she didnââ¬â¢t have any plans for marriage. To try to fill the need she had felt, Lillian chose nursing. She enrolled into the New York Hospital Training School for Nurses, and after finishing the two-year program at the Nursing School in 1891, she took a position at the New York Juvenile Asylum.
Sunday, November 10, 2019
Consolidating the Directv Acquisition
Case 7: News Corp. in 2005, Consolidating the DirecTV acquisition The case focuses on the acquisition and consolidation of DirecTV by media mogul Rupert Murdochââ¬â¢s News Corp. After the acquisition, Murdochââ¬â¢s content-and-distribution empire spanned four continents, with more than 26 million subscribers. Murdoch, News Corp. ââ¬â¢s chairman, had ambitions to build one of the most powerful media companies in the world. He aspired to have a balance between subscription and advertising revenue, the best mix of content and distribution and the widest geographic spread in the world.There are a various aspects of the general environment firstly; the political/legal aspect of the general environment was initially most significant in the purchase of DirecTV by News Corp. FTC concerns of potential market concentration issues if EchoStar purchased DirecTV forced the two to drop discussion, paving the way for News Corp. ââ¬â¢s purchase of DirecTV. Technology is probably the most important current aspect of the general environment.Secondly, Demographic segment Growing markets worldwide for various communication and entertainment media content. Thirdly, Socio-cultural segment largely due to rapid changes in technology, as well as increased overall globalization efforts, consumers are becoming increasingly sophisticated in the quality and type of communication and entertainment that they expect. Communication, news and entertainment provided by DirecTV and similar services have become viewed as nearly a necessity in a modern world.Fourthly, Economic segment while general economic conditions and level of economic development in a particular country or region may hinder some feasibility of these types of services, in general, they are needed and used by markets worldwide regardless of overall economic conditions. Fifthly, Political/legal segment The FTC, the governmentââ¬â¢s watchdog arm that oversees competition policy, is responsible for ensuring that cons umersââ¬â¢ interests are protected. If an industry is getting increasingly concentrated, the FTC can use antitrust laws to prevent further consolidation of the industry or break up previous consolidation.The FCC, the governmentââ¬â¢s watchdog arm that oversees the communications industry, is responsible for overseeing companies that sell communications products and services. Finally, Technological/global segment The 1980s and 1990s saw a worldwide race to deliver television programs to global markets. Cable operators invested in laying cables and beamed programming content through these cables into subscribersââ¬â¢ homes. Satellite television had a much wider reach, because satellites orbiting the earth sent the programming content directly into the home without the need for a cable connection.By 2000, cable had an important advantage over satellite in that it offered high-speed, two-way access, including phone capability. Satellite was still mostly one-way. However, it wou ld take cable companies years of multi-million dollar investments to upgrade to digital technology. DirecTV competes in a broadly defined media industry. Several sectors of this industry might include communications (e. g. telephone, internet, cable, satellite TV), news services, entertainment (e. g. , cable and network programming, movies, pay per view, gaming). There are various characteristics of the dominant industry.Market size is the most providers are regional or national competitors, depending on the facet of media in which they compete. Life cycle stage is growth. Growth rate is rapid. Scope of rivalry is fragmented in some respects, but rivalry is fierce among those that go head to head in specific sectors. Rivalry concentration is the media industry is dominated by large and powerful companies, but most of these are regional, national or multi-national players. Pace of industry change is the technology change is rapid in telecommunications and related fields, which drives significant rapid change in all aspects of media.Product/service differentiation is the consumers often have changing options for substitute products. Porter's five forces model of competition to the industry. Generally media is a very attractive industry with substantial growth opportunities, both domestically and internationally. Extensive economies of scope and scale are becoming increasingly influential in this industry and its related sectors. Threat of entry: new technologies fuel the constant threat of new entrants into some facet of content and/or delivery in the media industry. Bargaining power of suppliers: Bargaining power of suppliers is not significant here.Content suppliers must deal with DirecTV due to its massive size and market share. Bargaining power of buyers: Customersââ¬â¢ bargaining power is very significant, as consumers increasingly have choices of how and when they take delivery of content. Threat of substitute products: many potential substitutes exist, along with many ways to access content. Rivalry amongst existing competitors: Rivalry is fierce, as News Corp. had substantial content that cable providers needed for product scope, permitting premium pricing. Cable and satellite are constantly battling for new or switching customers.Critical success factors DirecTV must continue to be aggressive in its acquisition and implementation of new technology. They must also be keenly aware of customer tastes and preferences for content and delivery options. There are two types of resources Tangible and intangible. News Corp. ââ¬â¢s tangible resources are its global satellite platform and its strength in both content and distribution, as well as an impressive set of fast-growing networks such as Fox News, Fox Sports, National Geographic and Speed Channel and satellite distribution in Europe, Asia and Australia. Before its acquisition of DirecTV, News Corp. as a global company, but had no direct broadcast satellite presence in the United States. DirecTV had three primary components at the time it was acquired by News Corp. , all of which appear useful for expanding News Corpââ¬â¢s scale and scope of offerings: it was the largest US provider of direct broadcast satellite, giving customers access to hundreds of channels of digital-quality video and audio programming; PanAmSat owned and operated 25 satellites that covered 98% of the worldââ¬â¢s population; and broadband satellite networks and services were provided to both consumers and enterprises.Intangible resources an aggressive corporate culture used to dealing in the high-pressure, ever-changing media industry, which fuels significant interest in developing scope and scale of product content and delivery. There are unique internal resources and capabilities of DirecTV that played a prominent role in Murdochââ¬â¢s decision to acquire DirecTV. DirecTV was the first entertainment service in the US to deliver all digital-quality multi-channels TV programmin g through an 18-inch satellite dish. In 2003 DirecTV had 12. million subscribers, 10. 7 million of which were DirecTV subscribers, and the remaining receiving DirecTV service from members and affiliates of the National Rural Telecommunications Cooperative. This unique capability potentially provided Murdoch with a vast American market and the ability to shake his programming from the seeming stranglehold of American cable companies. The rivalry like between News Corp. and its competitor(s) during the acquisition of DirecTV. In September 2000, Murdoch offered $22 billion for a 35% stake in DirecTV.In Feb 2001 Hughes stock declined, and Murdoch reduced his bid for a 30% stake. As News Corp. negotiated with Hughes Electronics for the sale of DirecTV, the progress was very slow. In 2001, EchoStar surprised many by making an unsolicited bid to buy Hughes for $30. 4 billion in stock and $1. 9 billion in assumed debt. EchoStar was in the US satellite pay-tv market and a combined DirecTV an d EchoStar would control as much as 92% of that market. Murdoch used this knowledge to lobby intensely to block the merger on antitrust grounds, and DirecTV and EchoStar dropped their deal.In April 2003, News Corp. acquired GMââ¬â¢s 19. 9% stake in Hughes and a further 14. 1% from public stakeholders. The feature of the external environment does News Corp. use to fend off its rivals. Murdoch used a US government regulatory body, the FTC, to make sure that his rival EchoStar did not get a chance to buy DirecTV. He forced the issue to the FTC that combination of DirecTV and EchoStar would create a monster that would control 92% of the market. Once the sale to EchoStar was blocked, News Corp. went forward and made a successful bid for the purchase of DirecTV.The News Corp. integrates the newly acquired DirecTV into its global strategy. DirecTV was immediately re-structured. Labor disputes were settled and half the employees were retrenched. DirecTV became increasingly focused on cor e business, selling its 80% stake in the satellite launch business for $2. 5 billion, and its set-top-box manufacturing and XM Satellite Radio. The proceeds of these sales were used to pay about $1. 4 billion for rural satellite companies that had 1. 5 million subscribers, dramatically increasing market share.The factors inhibit News Corpââ¬â¢s strategy from delivering its full potential. In order to close the DirecTV deal, News Corp. had to make some concessions: (1) Since the FCC had already banned large cable operators from discriminating against rival programmers, DirecTV could not use its muscle power to its full advantage (2) News Corp. had to submit to arbitration if cable operators accused it of using its popular channels as bargaining tools. These restrictions were temporary, as they expired within six years.
Friday, November 8, 2019
buy custom Censorship essay
buy custom Censorship essay With the current advancements in all sectors of the society, there is a need to control circulation of ideas that may be harmful to the lives of people in the world. Censorship is managing of information that flows in the society. Through history, censorship has been a subject of dictatorship. The 21st century society has achieved this, as it was able to examine all sorts of information that reaches the entire society through any means. According to Bauder (87), there are various reasons that justify the existence of censorship in the society. Some of the reasons include the removal of obscene and blasphemous ideas, or ideas dealing with treason. It is evident that not all forms of censorship are equal, and not all forms have the same origin. The sources of censorship include self-government or external sources. To get further knowledge on censorship and the need to censor, it is important to remove the label that has been attached to it. An individual should realize that essentialit y of censorship dates back to the ancient times when all societies had rules and regulations that governed their speech, dress code and relationships. These rules played a critical role in ensuring that the society lived in peace throughout its history. Censorship has been used in various countries of the world to foster various ideologies. Plato, a Greek philosopher, was the first to introduce censorship of various ideas. Censorship gives room for punishment of individuals who in one way or the other utter evil speeches in public. Abrams (165) says that there is an extent to which campus censorship infringes on the First Amendment, an act that grants freedom of expression. History It has been evident that sometimes free speech given by students clash with the schools main objective of keeping control over the way the general student populace is educated. Des Moines, a renowned thinker, together with the self-regulating school district played a crucial role in availing students liberties granted by the First Amendment. The act provided that a student was free to carry symbols, such as an arm band, to protest against injustice in the society, such as the Vietnam War. Various cases forwarded by the school community pointed out the need for censorship on campus. In Hazel Wood, a school principal was granted a chance by a court of law to extract some articles from the schools publication because to him the information was harmful to schools continued role in the educational process. Some parts of these articles had information on teenage pregnancies in schools, as well as emotional feelings of students whose parents have divorced (Souza, 254). The court proclaimed that a schools publication is not a site for individuals to post their opinions on public affairs. They gave the school officials the authority to determine the kind of information that should be published in school journals. There was also a ruling three years after the Hazelwood ruling that was based on Planned Parenthood. A school had sought public commercials from businessmen around the school community. The adverts included the one from Planned Parenthood. Planned Parenthood filed a suit against the principal for his refusal to place their advert. Here, the court decision favoured the school citing the fact that a school publication is not a forum where the public can post their adverts. The school officials, therefore, were free to decide which posts should feature in their publications. From these rulings, the general public saw that school districts apply some form of censorship. Public universities in the US apply various rules and regulations to control freedom of speech. To them, students should seek to use proper choice of words so as not to harm or disrespect others. Though freedom of speech is a fundamental right provided in the constitution, some public colleges and universities may prohibit the use of certain words that provoke violence or threats. They may also forbid the use of obscene language and defaming statements. However, they prohibit all these without actually altering the First Amendment Act. There are various cases that have arisen contesting some of the prohibited acts, also termed as unconstitutional. At the University of Michigan, censorship prohibited various discussions on biological differences between opposite sexes and different races. In this case, the court ruled that the universitys constitution had not infringed on the First Amendment Act, since students were allowed to speak about various other things. At Wisconsin, a student was taken to court for yelling rude remarks at a woman. The universitys clampdown on the freedom of speech, in this case, was also overruled as being excessive (Wilson, 145). Years after the Fraternity Brothers were taken to court for dramatization of an ugly woman contest, it was found that the accusation of the Fraternity Brothers was against the First Amendment Act. This is because they did not take them to court for their conduct on stage, but because of the message that was conveyed. To tem, this was against their policies. Campus censorship also includes the use of the Internet. In most universities, students are not allowed to view pornographic videos. Students use the Internet as their main tool of gaining or spreading information. The censorship of communication through the Internet is slowly taking root in various universities of the world. This despite the fact that reality is a complex exercise, which may involve all stakeholders in the field of networking. In most campuses, it is a criminal offence to view or distribute pornographic materials. Some sections of the universities constitution are being altered to include the law that governs such kind of materials. Students on campuses are described as the most powerful informative population. They have the ability to participate in various forums where they engage in conversations over various matters. The First Amendment allows for freedom of expression, and that is what most students use during their public forums. They take the opportunity to air their grievances and also seek for various needs that they feel should be addressed. Introduction of censorship on campuses is detrimental to this. It plays a crucial role in denying students freedom of speech. Campuses have used the presence of censorship as an opportunity to deny students a chance to air their grievances. Pros of Censorship There have been various disputes over whether prohibiting information is a good or bad idea on campuses. There are several advantages of censorship of information. First, censoring pornographic materials is advantageous in avoiding corruption of young minds. Students on campuses are young, and even if they are able to control their behaviours, pornography puts them at a great risk of engaging in sexual immorality. Human beings can be consumed by emotions, and some may want to try what they see in pornographic movies. Some of pornographic materials are just graphics, but people may think that they are real and they will end up indulging in these activities. Censorship, therefore, prevents such kind of behaviour. In a culturally diverse community, censorship plays a key role in maintaining peace and unity. The USA is a country where people of various races live, and it is important to maintain multicultural policy, which aids in preventing conflicts between certain communities. Words are commonly known to hurt, especially when they are directed to members of a minority group in society. The main focus of multiculturalists is to nurture and not to separate a minority group of students. They strive to do away with racism and gender biases in the society, as well as in the minds of the minority population. Censorship of any information that is detrimental to the task of multiculturalists plays an essential role in nurturing minority groups in society. Censorship helps in preventing public display of impertinence towards an individual or a group. On campuses, students may dislike a particular idea that has been proposed by a leader. If it is against their wish, they may decide to be opposed to it, and even go so far as to disrespect the authority. For example, in CaliforniaStateUniversity a student was putting up fliers advertising a speech that was supposed to take place the following night. The speech was intended to incite hatred against some educational programs for black students in the USA. This was to be done by launching a book written by Weaver which analysed the programs and linked them with slavery. When he arrived at the multicultural centre, the black students persuaded him not to deliver the speech by claiming that the material was abusive and discourteous. They quoted the fact that the flyer was a reason to alarm the police, as there was a possibility of demonstrations. Censorship also guards the mores of society (Arthur, 56). Some campuses have introduced rules and regulations to govern the behaviour of students towards their teachers, dress codes and drug abuse. In most protestant universities, there is a specific dress code for students attending classes. Students are not allowed to show any kind of disrespect towards their teachers or use drugs in a classroom environment either. These regulations have made campuses a place where genuine morality is preserved. This is because students being admitted to campus are not chosen based on their religious beliefs or the fact that they are drug addicts. Censorship of this kind helps maintain order within an institution and ensures that learning takes place as intended. Cons When topics related to sex are fully censored, it becomes very hard to instruct students and young people about the dangers posed by HIV/AIDS. In most institutions, sex-related topics are censored. According to their administration, availing this information makes the students vulnerable to the issues discussed, which include engaging in sexual activities at an early age. It shoulld, therefore, be noted that HIV/AIDS spread fast on campuses, because students have not been given an opportunity to consider the possibilities of engaging in safe sex. Abortion cases among campus students are also on the rise. This statistics are attributed to censorship of sex-related information in the institution. Censorship has encouraged the emergence of a number of dictator-minded individuals in educational institutions. They use this opportunity to promote themselves by using eloquence, and thus doing away with any negative image they may have. For example, at the CornellUniversity, an article that, according to the university administration, was defamatory was censored. The article spoke about the blacks as the most violent group of people as opposed to their white counterparts. The article also cited instances of black students being violent in the university, and issued a warning to the white students to be careful. It was seen to be promoting the acts of some of the leaders of the university administration and student governance, who were trying to silence the conservatives. A professor of African-American History at the CornellUniversity saw this article as a tool that some of the leaders use to justify putting the blacks in prison. The professor saw this act as an effort to infringe on the freedom of speech. He also linked it with the infringement of the First Amendment Act with respect to the blacks. According to Oberst, it is clear that, in the American society, blacks are not given an opportunity to defend themselves against any form of violent activity that they are engaged in. When black and white students are charged with the same offence, the possibility that the black student will be jailed, and the white student let free is high. High-ranking academic officials also use censorship to protect their reputation. In an institution, where a member of administration wants to impose some unfavourable rules that were not initially in the constitution, censorship becomes a powerful tool to foster these acts. Silencing students ensures that they do not get an opportunity to engage in public forums and discuss the pros and cons of the idea. Those who want to initiate discussions are silenced by their counterparts for fear that they will be charged with incitement and going against the constitution. Censorship infringes the First Amendment Act, which gives students freedom to air their grievances. The act stipulates a clear need to encourage freedom of expression for students. Censorship has turned most of the universities forums and publications into sites, where information regarding student affairs only is allowed to be discussed or published. There are students, who have suffered broken families, sexual assault and HIV/AIDS. This group of students would like to share what they are going through with their peers. Censorship prevents them from doing so by making them vulnerable. They then turn to drugs as an option to suppress their negative feelings. Censorship also infringes on the freedom of press. When a university publication is given restrictions on where to get advertisements from, this restricts their freedom of speech. Censorship is closely linked with dictatorship. It is a tool used by those in power to rule the populace. For example, when there is censorship of blas phemous information against an individual in power, it means that the ignorant few will never know the evils that the individual is carrying out. The society will then continue to rot under the leadership of dictators. Censorship on campus kills creativity. The case of Fraternity Brothers, who were charged with dramatizing a pageant contest, reflects the extent to which restrictions on campus harm creativity. Censorship gives rise to and conceals human rights maltreatment. Since people are not permitted to air their views on a certain public matter or private offences, their rights are infringed. When a university student wanted to enlighten the black students on the effects of some programs that, to him, are linked to slavery, the act was termed as unconstitutional and going against the multicultural policy of the institution. Conclusion Censorship on campus has turned out to be enveloping and invasive. It focuses mainly on highly inconsequential offenses (Roleff, 143). This does not imply that censorship on campus did not exist in the past. Censorship is a persistent issue; the urge to prevent the airing of information that is harmful or you fear might affect your reputation is almost primordial. This particularly true of individuals holding high offices and those involved in politics. There are some benefits of censorship but its negative effects have a harmful consequence for the student fraternity at large. It is essential for all the stakeholders involved to discuss the effects of censorship in high academic institutions and the extent to which it should be carried out. This is done keeping in mind that campus students are of age, and they have a right to knowledge on varying issues. Censorship is bad as it infringes on the freedom of expression. Buy custom Censorship essay
Wednesday, November 6, 2019
Why had Henry failed to achieve his aims in foreign policy by 1529 Essays
Why had Henry failed to achieve his aims in foreign policy by 1529 Essays Why had Henry failed to achieve his aims in foreign policy by 1529 Paper Why had Henry failed to achieve his aims in foreign policy by 1529 Paper Essay Topic: History Henrys aims since he came to the throne in1509 was glory, he thought he could get this through vast amount of money. Henry at the time was almost broke and therefore needed glory quickly, and the way Henry came up with to make money and gain glory was via war. He needed to attack France but he had no money, Wolsey raised the funds and so became very close to Henry and he went on to gain glory. Although Henry was satisfied with the war he still wanted more glory and to be a dominant figure in Europe. Unfortunately Henry had not achieved his aims by 1529, this was due to a number of reasons, one being that the other powers in Europe were much bigger and grew faster than Henry so he could not keep up. One important reason for Henrys failure to achieve his aims were a number of set backs during 1509 and 1529. One of these set backs comes as early as 1514 where even though Henry has won the war between France and England he is still in essence broke, this makes it hard for Henry to have any impact on foreign politics and does not put him in a good position to go to war again. Henry gets an opportunity to become a leading figure in Europe in 1518 with the Treaty of London, but unfortunately the Turkish never really attack so Henrys power goes to waste. In 1523 Henry is on course for taking over France (being only 50 miles outside Paris with 10,000 troops) but his alliance breaks down and he is forced to retreat. This shows that he was close to being a dominant force in European politics but just missed out due to this major setback. Henrys lack of military power was another thing that set him apart from the rest of Europe. Due to the fact that Henry was not as big as the other countries and that he could not pay his soldiers as much Henry simply did not have as bigger army as other dominant powers. This depicted Henry as weak to his fellow leaders, thus making him seem unimportant and not worth taking too much notice of. Wolsey was one of the reasons that Henry did not achieve his aims, this is because however much Wolsey helped Henry he would always be against him due to his want of the papacy. This meant that Henry never got what he quite wanted, and his ideas were always slightly adjusted, thus never giving Henrys desired outcome. The relationships within Europe were another reason Henrys aims were never accomplished, Wolsey would make alliances but Henry would regularly fall out with the other leaders splitting up the alliances. For example in 1516 Henry weakens his alliance with the Empire by falling out with Max. The traditional view of France as the enemy meant that Henry never really had an alliance with France. The major blow was when Wolsey missed out on the France + Empire + Pope Treaty, this showed Henry was not a dominant as he was not respected enough to be asked into the alliance. Henry was competing against very powerful kings in Europe, thus finding it hard to make an impact on foreign politics. Charles the 5th controlled most of Europe making him very powerful militarily and dominating most of Europe. Whereas Henry had England which was relatively small and had a small population. Francis 1st has vast amounts of money that gave him respect and the ability to do things he wanted such as build an army or show off his wealth (the field of cloth and gold). Whereas Henry was almost broke and struggled to raise funds to go to war. In conclusion Henrys lack of power and dominance and the fact there were strong leaders in Europe meant he was not taken as seriously as the others, and he could do nothing to make them listen, as he simply did not have the resources to challenge them. The other greater powers meant Henry had no chance of coming into line with them as he could not grow in power quicker than them, especially with the money he had. If Henry had gone with Wolseys ideas totally then Henry may have found his glory in peace rather than war, and in turn saved more money. This would have meant he could have executed his foreign policy better. Unfortunately this never happened therefore meaning that Henry never achieved his foreign policy aims by 1529.
Monday, November 4, 2019
Coursework. Immunoprecipitation Technique Coursework
. Immunoprecipitation Technique - Coursework Example The basic idea behind this technique is the separation of one single protein from a mixture of proteins. This could give us the idea of several characters of a protein such as its relative occurrence in a solution, itââ¬â¢s up and down regulation as well as its affinity for a specific antibody. Technique/Methodology Usually the process of immunoprecipitation can be completed in two ways: in sequential or in one step. Commonly the antibody on which the required protein is supposed to be attached is immobilized on a solid support such as beads. This solution containing the immobilized antibody and the beaded support is then incubated with the solution that contains the protein mixture containing the required protein. Incubation allows the specific protein to bind to then antigen and form a complex. This complex can then be separated from the solution and studied with different techniques such as ELISA or Western Blot according to the requirements. (Pierce Biotechnology 2011) (Pierce Biotechnology 2011) The diagrams courtesy of ââ¬ËPierce Biotechnologyââ¬â¢ show the process of immunoprecipitation starting from preparation of a solid support along with the antigen to the incubation and the formation of the antigen-protein complexes till the precipitation of the required protein. Types There main types of immunoprecipitation are: 1. ... pitation: Same as Chromatin only difference lies in the detection of RNA binding proteins Uses Immunoprecipitation has been useful in many aspects such as, It has enabled the scientists to know the activation of the proteins, their molecular weight and also separate some protein binding molecules too. This technique has also been helpful in detecting the abundance and activity of a protein. Protocols After collecting the required number of cells and washing them in ice cold PBS, the solution was spinned at 1000g at a temperature of 4 degrees. This would help in separating the supernatant fluid. The next step involves the resuspension of the pellet that contains the cells in an ice cold buffer after which the cells are lysed by centrifuge. The supernatant fluid is removed after spinning it again at 13000g at the same temperature for fifteen minutes. Bradford assay is then used to measure the quantity of the protein after the supernatant fluid is removed by spinning briefly. This super natant free solution is then incubated in a cold room with the required amount of specific antibody solution. After the addition of Protein A or G beads to the tube it is again incubated for an hour and then spinned briefly again so that further supernatant is removed. This beaded fraction is then washed with ice cold buffer and spinned to remove the supernatant and the resultant solution is kept for further analysis. BCL-2 Proteins Bcl 2 family proteins have been identified to play a major role in the process of cell death i.e. apoptosis. These proteins play both anti and pro apoptotic roles. Some members of the family are supposed to increase while some are supposed to decrease this process of apoptosis. In this project the interaction of Bcl 2 family proteins with PUMA have been
Friday, November 1, 2019
The Attributes that makes the Best Corporations Essay
The Attributes that makes the Best Corporations - Essay Example Managers are people who get the work done through other people. Managers use their conceptual, human, and technical skills to perform four management functions of planning, organizing, leading, and controlling in all organizations - large and small, manufacturing and service, profit and non-for-profit. But not all managers' job is the same. Managers are responsible for different departments, work at different levels in the hierarchy, and meet different requirements for achieving high performance. All managers must contribute to planning, organizing, leading, and controlling in their organizations - but in different amounts and ways. Top managers are at the top of the hierarchy and are responsible for the entire organization. They have such titles as president, chairperson, executive director, chief executive officer (CEO), and executive vice-president. Top managers are responsible for setting organizational goals, defining strategies for achieving them, monitoring and interpreting the external environment, and making decisions that affect the entire organization. They look to the long-term future and concern themselves with general environmental trends and the organization's overall success. Among the most important responsibilities for top managers are communicating a shared vision for the organization, shaping corporate culture, and nurturing an entrepreneurial spirit that can help the company keep pace with rapid change. Today more than ever before, top managers must engage the unique knowledge, skills, and capabilities of each employee. Middle managers work at middle levels of the organization and are responsible for business units and major departments. Examples of middle managers are department head, division head, manager of quality control, and director of the research lab. Middle managers typically have two or more management levels beneath them. They are responsible for implementation the overall strategies and policies defined by top managers. Middle managers are concerned with the near future, are expected to establish good relationships with peers around the organization, encourage teamwork, and resolve conflicts. Recent trends in corporate restructuring and downsizing have made the middle manager's job difficult. Many companies have become lean and efficient by laying off middle managers and by slashing middle management levels. Traditional pyramidal organization charts are flattening, allowing information to flow quickly from top to bottom and decisions to he made with the greater speed necessary in today's highly competitive global marketplace. Nowadays there is a tendency of middle management shrinking. For example, Eastman Kodak recently cut middle management by 30 percent and reduced its middle management levels from seven to three. The Medical Systems Group at General Electric cut middle management by 33 percent. These cuts have improved the efficiency and performance of many corporations via improved responsiveness to customers, speed in new product development, and increased profits. The decline in middle management and the simultaneous improvement in corporate efficiency are
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